منابع مشابه
Microbial system for polysaccharide depolymerization: enzymatic route for xanthan depolymerization by Bacillus sp. strain GL1.
An enzymatic route for the depolymerization of a heteropolysaccharide (xanthan) in Bacillus sp. strain GL1, which was closely related to Brevibacillus thermoruber, was determined by analyzing the structures of xanthan depolymerization products. The bacterium produces extracellular xanthan lyase catalyzing the cleavage of the glycosidic bond between pyruvylated mannosyl and glucuronyl residues i...
متن کاملElectrospinning of Nanodiamond-Modified Polysaccharide Nanofibers with Physico-Mechanical Properties Close to Natural Skins
Electrospinning of biopolymers has gained significant interest for the fabrication of fibrous mats for potential applications in tissue engineering, particularly for wound dressing and skin regeneration. In this study, for the first time, we report successful electrospinning of chitosan-based biopolymers containing bacterial cellulous (33 wt %) and medical grade nanodiamonds (MND) (3 nm; up to ...
متن کاملXanthan chain length is modulated by increasing the availability of the polysaccharide copolymerase protein GumC and the outer membrane polysaccharide export protein GumB.
Xanthan is a polysaccharide secreted by Xanthomonas campestris that contains pentameric repeat units. The biosynthesis of xanthan involves an operon composed of 12 genes (gumB to gumM). In this study, we analyzed the proteins encoded by gumB and gumC. Membrane fractionation showed that GumB was mainly associated with the outer membrane, whereas GumC was an inner membrane protein. By in silico a...
متن کاملPolysaccharide lyase: molecular cloning, sequencing, and overexpression of the xanthan lyase gene of Bacillus sp. strain GL1.
When grown on xanthan as a carbon source, the bacterium Bacillus sp. strain GL1 produces extracellular xanthan lyase (75 kDa), catalyzing the first step of xanthan depolymerization (H. Nankai, W. Hashimoto, H. Miki, S. Kawai, and K. Murata, Appl. Environ. Microbiol. 65:2520-2526, 1999). A gene for the lyase was cloned, and its nucleotide sequence was determined. The gene contained an open readi...
متن کاملProduction of Protein-Polysaccharide Fibers Containing Omega-3 Fatty Acids Using Electrospinning Method and Determination of Optimal Process Conditions with Response Surface Methodology
Background and Objectives: Omega-3 fatty acids are very important in the human diet for the prevention of diseases. Materials & Methods: In the present study, the effect of whey protein concentrate (5, 6.67, 10, 13.33, 15%) and guar gum (0.1, 0.25, 0.55, 0.85, 1%) were evaluated on the qualitative properties of fish oil emulsion and electrospun fibers. Results: Increasing concentration of whey...
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ژورنال
عنوان ژورنال: Macromolecular Materials and Engineering
سال: 2017
ISSN: 1438-7492
DOI: 10.1002/mame.201700067